Simultaneous BOLD fMRI and two-photon microscopic imaging on mice brain at 16.4T

While BOLD-based fMRI can non-invasively map whole brain activation and connectivity in humans and animals, the BOLD signal provides only an indirect measure of neural activity, and its cellular and neurophysiological origins remain not fully understood. We have developed a multi-scale neuroimaging modality allowing simultaneous fMRI and two-photon microscopic imaging (TPMI) on mice brains at UHF (16.4T). With the virus injection of GCaMP6s into the mouse brain, for the first time, we have successfully obtained functional MR images of the mouse brain and the neuronal calcium signals at layer II-III of the right S1 cortex simultaneously at resting state.