One‐pot synthesis of 1, 2‐dihydro [1,2,4] triazinium salt by copperassisted unprecedented cyclization reaction: Application in protein binding

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Copper catalyzed intramolecular annulation of 2-((2-benzylidene-1-phenylhydrazineyl)methyl)pyridine derivatives was described. It was found that Cu(II) is reduced under the reaction condition to Cu(I). Synthesized 1,2-dihydro [1,2,4] triazinium salt showed fluorescence activity in the solid state. On treating with base, an instant increase in fluorescence was observed. A detailed physicochemical assessment underscored the robust DNA-binding capabilities of the [1,2,4]triazinium cationic species (C1-C3) via intercalative mechanisms. Notably, binding assays with BSA accentuated the heightened nucleic acid affinity of these cationic species.