Figure 1 from Tumor Vessel Normalization via PFKFB3 Inhibition Alleviates Hypoxia and Increases Tumor Necrosis in Rectal Cancer upon Radiotherapy

3PO affects normal epithelial and colorectal cancer cell proliferation and viability in a concentration-dependent manner. A, xCELLigence assay performed with HCT-116, HT-29, SW-1463, SW-837, HUVECs, and RPE-1 cells. Real-time cell analysis following treatment with different concentrations of 3PO over a period of 96 hours. Dotted lines indicate the fitted saturation curve in logarithm, n = 3 independent experiments. B and C, CellTiter-Blue assay performed with HCT-116, HT-29, SW-1463, SW-837, HUVECs, and RPE cells treated with different concentrations of 3PO for 24 and 48 hours. D and E, LDH assay performed with HCT-116, HT-29, SW-1463, SW-837, HUVECs, and RPE cells treated with different concentrations of 3PO for 24 and 48 hours. B–E, Data are displayed as means SD, ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. One-way ANOVA was used to calculate statistical significance, n = 3 independent experiments.