Figure 3 from PTP4A2 Promotes Glioblastoma Progression and Macrophage Polarization under Microenvironmental Pressure

In vivo phenotype associated with PTP4A2 modulation in P3 xenografts. A, Western blot analysis of Ctrl, PTP4A2-KO, and PTP4A2-OE P3 cells. B, Tumor volume assessed by bioluminescent imaging from orthotopically xenografted P3 spheroids (KO, Ctrl, and OE) over approximately 2 months after implantation. Kruskal–Wallis test followed by Dunn tests. n = 8 mice per group. C, Tumor size of P3 PTP4A2-KO, Ctrl, and PTP4A2-OE tumors. Mice were sacrificed at the same time point to compare the tumor area. n = 5 mice per group. D, Kaplan–Meier curves of survival (time to endpoint) of mice bearing KO, Ctrl, and OE tumors. Survival was not affected by the KO but was reduced by the OE. Log-rank test. The number of mice is indicated in the figure. E, TUNEL assay performed on sections from P3 Ctrl, PRP4A2-KO, and PRP4A2-OE tumors showing significantly more apoptosis in KO tumors compared with Ctrl and OE. Kruskal–Wallis test followed by Dunn tests. n = 5 mice per group.