Investigation of Electrical Impedance of Human Breast Cancer Cells and Osteosarcoma Cells

Nanostructures exhibit a high aspect ratio and volume-specific surface area, endowing them with unique physical and chemical properties distinct from conventional bulk materials[1]. This study aims to develop cell-based biosensors (CBBs) for the non-invasive monitoring of MCF-7 (ATCC-HTB-22) and U2OS (ATCC-HTB-96) cell conditions. Vertically aligned nanostructures, coated with indium tin oxide (ITO), were employed as sensing substrates for cell growth. A polystyrene cylinder, attached to the (3-aminopropyl) trimethoxysilane (APTMS)-modified ITO sensing substrate using PDMS, was utilized to create a cell culture chamber. Impedance measurements were conducted with an E4980A precision LCR meter (Agilent Technologies) at 1 V, covering a frequency range of 20 Hz to 2 MHz, with 201 sample points. We verified the establishment of a functional biointerface by seeding MCF-7 and U2OS cells and monitoring impedance changes, indicative of cell attachment and growth. In conclusion, we successfully adopted the APTMS-modified ITO-coated sensing substrate to measure the impedance of MCF-7 and U2OS cells. Our goal is for this CBB platform to significantly contribute to the advancement of cancer drug development. References A. Gao et al. , "Silicon-Nanowire-Based CMOS-Compatible Field-Effect Transistor Nanosensors for Ultrasensitive Electrical Detection of Nucleic Acids". Nano Lett. 11 , 3974-3978 (2011). Figure 1