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August 7, 2024ACS Synthetic Biology

Engineering a Sinorhizobium meliloti Chassis with Monopartite, Single Replicon Genome Configuration

Marcel Wagner Johannes Döhlemann David Geisel Patrick Sobetzko Javier Serranía Peter Lenz Anke Becker

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Abstract

Multipartite bacterial genomes pose challenges for genome engineering and the establishment of additional replicons. We simplified the tripartite genome structure (3.65 Mbp chromosome, 1.35 Mbp megaplasmid pSymA, 1.68 Mbp chromid pSymB) of the nitrogen-fixing plant symbiont

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Cite This Study

Wagner et al. (2024) studied this question.

synapsesocial.com/papers/68e5d34bb6db6435875692e6 https://doi.org/10.1021/acssynbio.4c00281

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