Formulation and evaluation of flavonoid derivatives’ as topical nano-emulgel to treat the wound healing in diabetic patients

Numerous approaches, including the utilization of phytochemical substances, have been developed to aid in the healing of diabetic foot wounds. Quercetine (Qr) and Silymarin (SM) are flavonoid components which have antioxidant activity and anti-inflammatory activity properties for burns and wounds healing. Both of them have poor aqueous solubility and permeability. This research aimed to prepare Qr and SM by incorporating with natural oil as nanoemulgel and evaluate its characterizations; since applying medication locally ensures patient compliance by delivering the medicine to the receptor site in an effective concentration at a low dose. The procedure had started by producing eight formulations produced by mixing the drugs with triacetin oil, tween 80 and transcutol P were used as oil, surfactant and co-surfactant, respectively. Then, starting the nanoemulsion evaluation and nanemulgel characterizations after choosing the optimum formula. The optimum formula according to droplet size (30 nm), zeta potential (-15 mV), PDI (0.287), drug content (Qr:99.32% and SM:97.89%), transmittance percent (99.9%), pH (6.1), viscosity (110.02 mPa.s), TEM (transmission electron microscopy; spherical nanosized droplet compatible with particle size analyzer), and in vitro release (around 100%) was F4. The later formula was incorporated into gel using carbopol 940 of 0.5, 1, 1.5 and 2% (N1, N2, N3 and N4, respectively). Formula N3 was the optimized produced nanoemulgel as it had better characteristics than others. In conclusion, Qr and SM were successfully formulated as NE. The optimum formula was formulated as nanoemulgel. The in vitro release showed successfully sustained release of the drugs.