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August 6, 2024Open Access

Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanning

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Authors

CBCaroline BenzLMLars MaasenLSLeandro Simonetti

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Overview

Benchmarking study demonstrates multiplexed deep mutational scanning identifies short linear motif binding determinants across viral and host proteins, highlighting tools for antiviral peptide design.

Key Points

  • Multiplexed deep mutational scanning identified key binding determinants across 19 coronavirus interactions, yielding an engineered peptide with potent antiviral activity.
  • Assessment using a multiplexed phage display protocol evaluated eleven peptide binding domains, confirming known determinants while uncovering an extended talin-1 PTB motif.
  • Highlights how high-throughput deep mutational scanning maps critical short linear motifs, providing a versatile framework to guide the engineering of high-affinity antiviral agents.

Cite This Study

Benz et al. (2024) studied this question.

synapsesocial.com/papers/68e5d477b6db64358756aa68https://doi.org/10.1101/2024.08.06.606761
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