Supplementary Video 1 from Anti-EGFR Antibody–Drug Conjugate Carrying an Inhibitor Targeting CDK Restricts Triple-Negative Breast Cancer Growth

Supplementary Video 1. Confocal Z-stack 3D video of ADC colocalization within lysosome clusters. Confocal microscopy video of a 3D reconstruction image of a live MDA-MB-468 cell showing spatial information of ADC colocalization within lysosome clusters after 24 hr of treatment (please also see Figure 4B). Cell represents treatment of 10 nM Alexa-Fluor-647 labelled ADC (magenta) for 24 hr, and stained with lysosome dye (orange) followed by Hoechst 3342 nucleus dye (blue).