Exploring the Role of Tp53 in Kidney Renal Clear Cell Carcinoma (Kirc): Expression Analysis, Promoter Methylation, and Survival Analysis and Mutational Analysis Through Bioinformatics Tools

In this study we focus on the expression, promoter methylation, mutation analysis and overall survival of MAPK1 gene in lung adenocarcinoma (LUAD) patients with the help of bioinformatics tools. Firstly the expression pattern of MAPK1 was analyzed in patient sample and compare with control group. The consequences showed that gene of interest are crucially down regulated in LUAD patient as compare to control group. Then to verify the result the expression of targeted gene was analyzed on the basis of other pathological attributes for example stages of life, sex, ethnicity and phases of cancer. Remarkable deviation of MAPK1 gene expression was detected in LAUD sample versus normal samples which emphasizes the pathological importance of gene expression in LUAD patients. When we analyzed the promoter methylation of MAPK1 gene in LUAD patients versus normal group a slight hypo methylation was found that shows the epigenetic regulation of target gene. Subsequently KM plotter tool was used to analyze the overall survival (OS) rate of patient with the expression of MAPK1 gene in LUAD sample in contrast to normal samples and found that overall good survival with low expression of the target gene. Mutation analysis of MAPK1 gene in LUAD sample by cBioPortal disclose that little genetic variation cause little dis-functioning of the gene. MAPK1 gene analysis point up the urgency of target gene as therapeutic and prognostic indicator in treatment of LUAD patients.