Extraction, Purification, Assay, and Antimicrobial activity of Bromelain from Pineapple

Pineapple- Ananas comosus (Morris) is a fruit of the tropics. Bromelain, an enzyme, is extracted from the stem, fruit, skin, pulp, and leaf. The crude enzyme having proteolytic activity was purified and precipitated. Protein estimation and its assay were done by Lowry’s method & GDU respectively. The molecular weight was determined by SDS-PAGE. Bactericidal activity was observed against both Gram-positive bacteria- Streptococcus and Gram-negative Bacteria-Salmonella by well method and tube method.