Analysis of antibiotic resistance in Enterococcus

Objective. To study the effect of antibacterial substances on bacteria Enterococcus isolated from the aquatic environment with anthropogenic load. Materials and methods. Study of 61 strains of Enterococcus isolated from water samples of the Vtoraya Rechka river, the Vladivostok territory, was conducted in the period from January 17, 2018 to September 27, 2020. The antibiotic resistance was determined using the disc diffusion test. Statistical data processing was carried out by the Statistica 10 program. Graphs were performed in Excel 10. Results. The study into susceptibility of 61 strains of Enterococcus to antibiotics revealed the majority of resistant strains to streptomycin (66%, n =40), 56% ( n =33) intermediate strains to erythromycin, 72% ( n =44) resistant strains to rifampicin, 87% ( n =52) susceptible strains to gentamicin, 25% ( n =15) intermediate resistant isolates, and 3% ( n =2) resistant isolates. Conclusion. The study of antibiotic resistance in strains of Е nterococcus isolated from the Vtoraya Rechka river showed high levels of rifampicin and streptomycin resistant strains. The population of enterococci strains isolated from the Vtoraya Rechka river appeared 41%-resistant to two antibiotics ( n =25), however, one strain proved multidrug resistance to six antibiotics. Resistance to a combination of streptomycin, rifampicin, and erythromycin antibiotics was most commonly detected.