Supplementary Figure S8 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy

Supplementary Figure S8. Overexpression of SB9(CAS) in ‘donor’ CD30.CAR with IgG1 spacer (CD30.Ig) TCRαβKO ATCs increases resistance to allorejection by ‘recipient’ Allo-T cells. A, Schematic of in-vitro MLR co-culture setup for (B-E), where ‘donor’ CD30.Ig TCRαβKO ATCs and ‘recipient’ Allo-T cells, which are CD30KO ATCs that were primed to recognize and kill ‘donor’ cells, were mixed at a 1:1 ratio. The MLR was performed in the presence of IL-7 and IL-15, both at 10 ng/mL. B-D, Cell counts, from a representative ‘donor’-recipient pair, of ‘donor’ T cells growing in monoculture (B), or ‘donor’ T cells (C) and ‘recipient’ Allo-T cells (D) in co-culture. E, The day 3 ‘donor’ cell death (%), where each point represents a unique ‘donor’-‘recipient’ pair and the bar chart represents the median (n = 6 per treatment group). P values were determined by one-way ANOVA, followed by Tukey post-test (E). *, P < 0.05; **, P < 0.01, ****, P < 0.0001.