Antimalarial plasmodione-based pro-photoaffinity probes for protein profiling ((pro-) AfBPP) based on the 3-benz(o)yl-6-fluoro-menadione scaffold were designed to be (photo)reduced and oxidized at the benzylic chain, to release photoreactive benzophenone-like groups. Under UV-irradiation, the AfBPP probe is released from the (pro-) AfBPP probe and applied to yeast and P. falciparum proteomes. Hence, covalent crosslinking of PD-AfBPP to its potential targets in yeast and P. falciparum proteomes occurs and enrichment of the probe-protein-biotin adducts is achieved by a CuAAC reaction between the probe-derived alkyne and the biotin azide. More details can be found in the article 10.1002/cbic.202400187 by Elisabeth Davioud-Charvet and co-workers. Image designed and created by Caroline Charvet and Elisabeth Davioud-Charvet.
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