Figure 2 from Inhibition of Notch4 Using Novel Neutralizing Antibodies Reduces Tumor Growth in Murine Cancer Models by Targeting the Tumor Endothelium

Treatment of human tumor xenografts with 6-3-A6 delays growth of tumors which express endothelial Notch4. A, IF staining of Notch4 (red) and CD31⁺ tumor vessels (green) in human tumor xenografts. Arrowheads indicate Notch4-expressing vessels (Scale bar = 200 μm). B and C, Tumor growth curves of subcutaneously implanted tumors from the indicated cell lines. B, MDA-MB-231 xenografts treated intravenously with 6-3-A6 (n = 5). C, Calu-6 human NSCLC xenograft tumors treated intravenously with 6-3-A6. All treatments started 12 hours after tumor implantation. Statistical analysis performed with two-way ANOVA for tumor growth curves, plotted as average ± SEM; *, P < 0.05.