Computational analysis of GCaMP fluorescence data in neuronal activity

The background and motivation of our research is to explore how to use computational methods to analyze GCaMP fluorescence data. GCaMP is a genetically encoded calcium indicator that can be used to observe the activity of thousands of neurons simultaneously using modern fluorescence imaging techniques. Our research includes the application of principles such as basic signal processing, statistical inference, hypothesis testing, and graph theory to help understand raw GCaMP fluorescence data recorded from awake, behavioral mice. The data set includes GCaMP fluorescence trace and correlation coefficient matrix among neurons. Our methods include high-pass filtering, Gaussian mixture model fitting and online active set method peak inference (oasis). By analyzing the correlation between neurons, we can understand the connection and centrality between neurons.