Surfactant-Free Synthesis of Melon Seed–Like CeO2 and Ho@CeO2 Nanostructures with Enriched Oxygen Vacancies: Characterization and Their Enhanced Antibacterial Properties

To overcome the poor antibacterial performance of cerium oxide (CeO2) nanoparticles at low concentrations, melon seed–shaped CeO2 (MS-CeO2) and holmium (Ho)-doped CeO2 (Ho@MS-CeO2) nanoparticles were synthesized using a simple precipitation method without the addition of any surfactants. The surface morphology, phase structure, crystallinity, Ce3+ and Ce4+ valence, lattice defects, and reactive oxygen species (ROS) production of both synthesized nanostructures were examined using different techniques, i.e., scanning electron microscopy (SEM), energydispersive X-ray (EDX), resolution transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), electron paramagnetic resonance (EPR), Raman spectroscopy, ultraviolet (UV) spectra, fluorescence spectra, and zeta potential (ζ). The results show that under certain stirring and aging temperatures, CeO2 and Ho-doped CeO2 nanoparticles with a melon seed–like morphology can be prepared in a short period. Both nanoparticles were tested as antiseptic agents against G+ and G– bacteria (E. coli and S. aureus), and the results confirmed that the Ho@MS-CeO2 nanostructures exhibited remarkable antimicrobial activity at a low concentration (0.5 mg/L) compared with the control group, which is attributed to the reversible conversion of Ce3+ and Ce4+ in the ceria crystal lattice, enriched oxygen vacancy, ROS species production, and positive surface charge.