Soil-Microbial Interactions in Rice-Loach-Shrimp Integrated Farming: Multivariate Analysis of Ecological Intensification

Ecological intensification of agriculture is crucial for sustainable food production, yet the complex soil-microbial interactions in integrated farming systems remain poorly understood. This five-year study compared a Rice-Loach-Shrimp (RLS) integrated system with conventional Rice Monoculture (RM) using advanced multivariate techniques, including correlation analysis, Principal Component Analysis, Random Forest modeling, and Structural Equation Modeling. The RLS system exhibited higher values for key soil health indicators compared to RM, although the overall correlation patterns were similar. Stronger correlations among soil organic matter, enzyme activities, and microbial populations were observed in RLS, suggesting a more tightly coupled soil ecosystem. Available phosphorus and soil organic matter were identified as primary drivers of microbial community structure. The RLS system showed a slightly higher mean pH and weaker correlations between pH and other indicators compared to RM, indicating potential altered biogeochemical processes. Complex cascading effects among soil properties, enzyme activities, and microbial populations were revealed in RLS. These soil health benefits persisted throughout the study period, demonstrating the potential of RLS as an effective ecological intensification strategy for sustainable rice production.