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Abstract Introduction : Club cell secretory protein (CC16) is reported to have multiple protective functions in airway diseases, including anti-inflammatory, immunomodulatory and antioxidant. This study aims to determine whether CC16 can repair lung injury caused by particular matter 2.5(PM2.5) exposure in asthmatic mice. Methods In the ovalbumin (OVA)-induced asthma murine study, 6-week-old male C57BL/6J mice were primary exposed to PM2.5 for 24 hours and following treated with CC16, Artery blood gas, lung function,histopathology and immunohistochemical staining were detected. The BEAS-2B cell line was primary exposed to PM2.5 for 24 hours and then treated with CC16 subsequently, fluorescence and electron microscopy, protein and RNA of inflammation and pyroptosis, and RNA Sequencing were detected. Results In the OVA-induced asthmatic mice after exposure of PM2.5 treatment with CC16 ameliorated PM2.5-induced lung tissue damage, respiratory acidosis and restore the increased airway resistance after PM2.5-exposed group, accompanied with the inhibition in the protein of inflammation and pyroptosis.Moreover, CC16 increased cell proliferation, ameliorated pyroptotic cell death induced by PM2.5 and inhibited the expression on the protein and RNA of inflammation and pyroptosis. Transcriptome analysis revealed that CC16 down-regulate genes associated with inflammatory adhesion, while up-regulating proliferation genes,like E-Twenty-Six-1. Conclusions CC16 could repair airway epithelium PM2.5-induced damage in asthma mice by up-regulating cell proliferation,inhibiting pyroptosis and imflammation, which it will been used as a novel therapeutic agent to alleviate the health risks of PM2.5 exposure in future.
Lin et al. (Wed,) studied this question.