Impact of Fixed Partial Dentures on Oral Microbial Flora and Gingival Health: An In Vitro Assessment of Salivary Microbial Profiles

Introduction The oral cavity hosts diverse microorganisms affected by factors like pH, temperature, and oxygen levels, influencing disease potential. Dentists manage oral diseases and improve aesthetics using durable restorations. Understanding periodontal response to crowns and fixed partial dentures (FPDs) is essential for effective treatment. This study aims to assess the impact of FPDs on periodontal health by comparing microbial colonies in individuals with and without FPDs. The hypothesis is that there will be no difference in microbial flora and gingival health between the two groups. Materials and methods This in vitro study utilized 40 salivary samples divided into two groups: 20 from patients with FPDs/crowns (Group 1) and 20 from patients without (Group 2). Unstimulated saliva was collected, diluted, and cultured on nutrient agar and Sabouraud Dextrose Agar to quantify anaerobic bacteria and