Abstract Objective Formoterol and salbutamol, β2-agonists, are listed in the Prohibited List of the World Anti-Doping Code International Standard, requiring both qualitative and quantitative analysis. To accurately determine the content of formoterol and salbutamol in urine, we developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Methods The samples were enzymatically hydrolyzed and extracted with organic solvents for liquid-liquid extraction, followed by purification and concentration. Qualitative and quantitative analyses were performed using tandem mass spectrometry with an electrospray ionization (ESI) source in multiple reaction monitoring mode. Results: The method demonstrated relative recovery rates between 90%-105%, matrix effects within 80–120%, and intra-day and inter-day precision below 15%. Conclusion This method is efficient, accurate, stable, and highly sensitive, fully meeting the detection requirements for the stimulants formoterol and salbutamol.
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Chen et al. (2024) studied this question.
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