Evaluation of DNA Content of Harvested Bone Prior to and After Cryopreservation at - 80o C for 6 Months in Bone Bank

This study investigates the impact of cryopreservation at -80C for six months on the DNA content of bone grafts, which is crucial for the success of orthopedic surgeries.Conducted at UCMS GTB Hospital, Delhi, bone samples from four patients were analyzed using Feulgen staining before and after the cryopreservation period.The results showed a complete absence of DNA content in all samples post -cryopreservation, highlighting a significant impact on the cellular viability of the grafts.This absence may affect the osteogenic potential and overall success of bone grafts.These findings underscore the need for further research to improve preservation techniques and enhance the functionality of bone grafts in clinical applications.