Figure S5 from Single-Cell and Spatial Transcriptome Profiling Identifies the Transcription Factor BHLHE40 as a Driver of EMT in Metastatic Colorectal Cancer

Figure S5. Spatial transcriptome and spatial trajectory of malignant EPCs_8 and CXCL1⁺ CAFs. (A–C) Spatial feature plot displaying possible expression distributions of BHLHE40, SMAD3, and SMAD4. (D) Cluster plot of 0–15 subgroups clustered by K-means. (E–G) Spatial feature plot displaying possible expression distributions of malignant EPCs_8, CXCL1⁺ CAFs, and SFRP2⁺ CAFs in another sample predicted by SPOTlight. (H) Spatial feature plot displaying possible expression distributions of BHLHE40 in another sample. (I) Possible spatial differentiation trajectory from malignant EPCs_8 to CXCL1⁺ CAFs in another sample.