Improvement of Reparative Regeneration of the Jaw Bone in Experiment

We conducted an experimental study on the effectiveness of the combined use of osteoplastic material Collapan-L with adhered MSCs to improve the processes of jaw bone regeneration in rats. The animals were divided into two groups: experimental and control groups of 50 animals each. Bone damage was induced in rats by drilling a defect with a dental bur in the area of attachment of the masticatory muscles in the angle of the mandible. We used only Collapan-L in the control group, and Collapan-L with adherent MSCs in the experimental group. During the cultivation process, statistically significant changes in concentration, depending on the day of cultivation, were observed for all cytokines: IL-1β (p<0.001 ANOVA), IL-2 (p=0.007 ANOVA), IL-4 (p=0.02 ANOVA), IL-6 (p=0.003 ANOVA), IL-8 (p=0.003 by Kruskal-Wallis test) and TNF-α (p<0.001 ANOVA). The dynamics of changes in the cytokines had a wave-like character. By the day 30 of the experiment, in animals with injected Collapan-L, the total volume of bone tissue in the regeneration zone was 50.14%, and in the Collapan-L + MSCs group it was 75.4%. The use of osteoplastic material Collapan-L with adhered MSCs is an effective combination for improving regeneration processes and reducing the inflammatory response of bone tissue.