P-343 Correlation Between Blood Lead Level (Bll), Vitamin D Level With Stress Oxidative Parameter: A Cross Sectional Study on Lead Exposed Workers in Java, Indonesia

Introduction In Indonesia, many Used Lead Acid Battery smelters operated by informal sector workers pose a potential health risk of metabolic and cardiovascular disorders through the oxidative stress mechanism and vitamin D deficiency due to chronic lead exposure. This study is aimed at analyzing the correlation of workers blood lead level (BLL), 25(OH)D level and Malondialdehyde (MDA) level as one of stress oxidative parameters, and Vitamin D level in Java Indonesia. Material and Method A total of 52 informal workers were consecutively selected from two regions in Java, one region with chronic and the other with non-chronic lead exposure. Venous blood samples were collected and analyzed for blood lead levels using ICP-MS, 25(OH)D with Elisa and MDA level using spectrophotometry with Will’s method. Result and discussion This study analyzes the correlation between groups with BLL <10 µg/dL and >10 µg/dL with the level of MDA (nmol/mL). We found that higher BLL has significantly lower MDA mean (BLL<10 mean 1.07, BLL>10 mean 0.59, P<0.01) on the region that has chronic lead exposure. MDA sub-analysis showed a significant difference in MDA mean between the two regions (1.31 vs 0.51, p<0.01). The analysis revealed a significant negative linear relationship between BLL and MDA (p < 0.01). No significant relationship was found between 25(OH)D and MDA (p > 0.01). Conclusion There is a significant negative linear relationship between MDA levels and BLL. We hypothesize the distinguish of this findings and other studies because the population with chronic lead exposure will lead to reduce physiological response to BLL compared to other region.