Simultaneous Quantification of Embelin, Piperine and Gallic Acid in Vidanaga Vati by High-Performance Liquid Chromatography

Background: Vidanga vati is a polyherbal formulation extensively used in Ayurveda as a remedy to treat fungal infections and warm infections. It mainly contains fruit parts of Embelia ribes, Piper nigrum, Phyllanthus emblica, Terminalia bellerica and Terminalia chebula. Aim: The present study aimed at developing a validated and reliable High-Performance Liquid Chromatography (HPLC) method for the simultaneous analysis of Embelin, Piperine and Gallic acid from Vidanga vati. Method: The method employed BDS Hypersil C18 column (250mm x 4.6mm, 5μm) as the stationary phase and Methanol: Phosphate buffer pH 2.4 (98:2 v/v) as the mobile phase to identify and quantify Embelin, Piperine and Gallic acid using HPLC. Results: Linear regression data for the calibration curves of standard Embelin, Piperine and Gallic acid showed a good linear relationship over a concentration range of 2-10μg/mL, 100-500μg/mL and 1000-5000μg/mL respectively. The percentage recoveries for Embelin, Piperine and Gallic acid were found to be in the range of 99.26-99.87%, 98.25-99.25% and 99.31-99.92% respectively. The method was assessed for accuracy, precision, selectivity and robustness. Conclusion: It was concluded that the developed HPLC method was efficient, simple, accurate and valid in the quantitative determination of Embelin, Piperine and Gallic acid.