Formulation Development and Evaluation of Andrographis Paniculata Cream: HPLC and HPTLC Analysis of Andrographolide From Extract and Cream Formulation

Andrographis paniculata is used for its numerous beneficial effects primarily for liver disorders.Currently, its only one semi-solid dosage forms are available in the market.It has been found to have antiallergic and anti-inflammatory effects.Hence, a cream formulation of Andrographolide leaves extract was developed.HPLC and HPTLC method was successfully developed and validated for determination of Andrographolide from extract and cream formulation.HPLC was performed using a mixture of Potassium dihydrogen orthophosphate: and acetonitrile as a mobile phase while for HPTLC a mixture of Tolune, ethyl acetate, methanol, and formic acid was used.Both methods were found to be robust, accurate and precise.