This study was conducted in. green house of the forestry science department nursery / Faculty of Agriculture and Forestry / University of Mosul for the period from 1/9/2022 to 1/7/2023. The main aim of this project was to study the effect of colchicine concentration (0 , 1000 , 1500 , 2000 , 2500 ) mg.L-1 and periods of Soaking (0 , 12, 18 , 24 ) hours and overlapping between them in the germination of tree seeds. Gleditsia triacanthos L. where used in the experiment the design of the complete random design CRD. Therefore, the results were indicated that the effect of colchicine concentrations and periods. Soaking has a significant effect in most of the studied traits, where the treatment of seed soaking with colchicine resulted in a concentration of 2000 mg.L-1 gave a significant increase in the content of leaves (carbohydrates , protein , chlorophyll a, chlorophyll b, total chlorophyll, moisture content, membrane stability) , also affected the periods of soaking 12 hours.in all the significantly studied qualities. Moreover, gave the highest rates of leaf content of ( protein , membrane stability). In addition, the 18-hour soaking period had a significantly impact and led to an increase in the content of the leaves of (carobohydrates, chlorophyll a, chlorophyll b, total chlorophyll) compared to the zero soaking period (control treatment), which gave the lowest rates and for all the studied qualities.
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alshahen et al. (2024) studied this question.