Anti-oxidant and Anti-bacterial activity of polyherbal formulation

The present work aimed to evaluate anti-oxidant and anti-bacterial activity of polyherbal formulation (gel) prepared using Tulsi, Aloe vera, Neem. It has been used for skin benefits since ancient times. Formulations were tested anti-oxidant activity by DPPH radical scavenging assay and anti-bacterial activity by disk diffusion Susceptibility Testing method. The anti-oxidant activity is exhibited in percentage inhibition. F1 formulation showed maximum percentage inhibition with increasing concentration as compared to standard ascorbic acid. The anti-bacterial activity is exhibited in zone of inhibition. The F5 formulation showed highest zone of inhibition (26.5 mm) against E. coli and zone of inhibition (26.0 mm) against Staphylococcus aureus which is an indication that F5 formulation is more effective on gram positive as well as negative bacteria.