The Antibacterial Activity of Tio2 Nanoparticles Prepared by Sol-Gel Method for a Group of Gram-Positive and Negative Bacteria

Titanium dioxide nanoparticles TiO2 NP were prepared by sol-gel method. TiO2 NP was identified and characterized using scanning electron microscopy (SEM), ultraviolet spectroscopy (UV-vis), Fourier transform infrared (FTIR), X-ray diffraction ( XRD) and atomic force microscopy (AFM). The SEM results showed an irregular spherical shape with different diameters (22.84-65.98) nm. The highest UV-Vis absorption was recorded at 345 nm wavelength. FTIR was used to find out the effective aggregates and the success of the process of forming TiO2 NP bands. The first centered between (450 – 800 cm-1), which is attributed to the patterns of stretching vibrations of the titanium oxide bond (Ti-O Vibrations). While the XRD peaks appeared at angles 2Ѳ (27.32°, 35.89° 39.03°, 41.02°, 43.88°, 54.09°, 56.38°, 62.43°, 63.77°, 68.67°, 69.41°, 76.11°) at Crystal Planes (110) (101) (200) (111) (210) (211) ( (220) (002) (310) (301) (112) and (202), (respectively, which corresponds to JCPDS standard tables), and this indicates the formation of rutile-type NPs TiO2. The results of (AFM) ranged between (6-7 nm) and the mean height (Z-mean value) was (8 nm). The effectiveness of NP TiO2 was tested at different concentrations (1, 0.75,0.5) mg/ml against a group of Gram-negative and Gram-positive bacteria:(Pseudomonas aeruginosa, Salmonella typhimurium, Bacillus subtilis, Staphylococcus aureus, E.coli) reached the highest zone of inhibition at a concentration of (1) mg/ml for Staphylococcus aureus, as the diameter of inhibition was (19.5).