New Approaches in Complex Treatment of Experimental Peritonitis

Resume. The aim of the work was to investigate the effect of probiotics as part of the complex treatment of fecal-induced peritonitis in rats. Matherials and methods. The experimental study was performed on 45 white, sexually mature male Wistar rats. Fecal peritonitis was modeled by a single intra-abdominal injection of a 10% suspension of syngeneic feces. Rats were randomly divided into 3 groups, 15 animals in each group. The treatment of rats of group I (main) consisted in washing the abdominal cavity with a 5% solution of probiotic disinfectant in a sterile 0.9% solution of NaCl 3-5 times, applying a probiotic disinfectant spray to the organs of the abdominal cavity after washing, and a gel with probiotics to the surgical wound with a thin layer after sewing it. To treat rats of the II group (comparison group), a solution of the sorbent “Enterosgel” in a sterile 0.9% solution of NaCl was used, with which the abdominal cavity was washed 3-5 times with exposure of the solution in the abdominal cavity for 2-3 minutes. Treatment of rats of the III group (placebo-control) consisted in washing the abdominal cavity with 0.9% NaCl solution 3-5 times. Results. In the first group (main), 10 (66.7%) rats survived, in which peritonitis was eliminated, 5 (33.3%) rats died. The animals that survived had a gradual regression of peritonitis. Peritonitis was eliminated in animals for an average of 6.5 days. The cause of death in 2 animals was pronounced peritonitis, 3 animals died during the experiment not from the progression of peritonitis. In the II group (comparison group), 8 (53.3%) rats survived, in which peritonitis was eliminated, 7 (46.7%) animals died. Peritonitis was eliminated in animals for an average of 8.5 days. 4 rats died of severe peritonitis, peritonitis was not pronounced in 3 animals at autopsy. All animals of group III (placebo-control) died of severe peritonitis within 1-8 days from the start of treatment. Conclusions. Probiotic solutions are effective for the rehabilitation of the abdominal cavity with peritonitis, which has been proven by experimental research and opens a new era in the treatment of peritonitis.