Microbiological justification for a combined approach to antimicrobial therapy for periodontal infection associated with periodontopathogenic anaerobes

Relevance. Despite the broader indications for the use of fluoroquinolones and imidazoles in dentistry, particularly in the complex treatment of periodontitis, the sensitivity of the principal causative agents of periodontitis, specifically anaerobic periodontopathogens, remains inadequately studied. Purpose. Is to justify the use of a combination of fluoroquinolone (ciprofloxacin) and nitroimidazole (tinidazole) chemotherapeutic agents using modern in vitro culture monitoring methods. To provide a rationale for the use of a combination of chemotherapeutic agents from the fluoroquinolone group (ciprofloxacin) and nitroimidazoles (tinidazole) utilising contemporary in vitro culture monitoring methods. Materials and methods. The study employed the method of automatic programmable bacterial cultivation in the "RTS-1" bioreactor (Biosan, Latvia) in vitro. The bacterial strains examined included S. consellatus , P. gingivalis and F. nucleatum . Results. The experiments demonstrated that the combined application of drugs from the fluoroquinolone and nitroimidazole groups (using the antibacterial complex Cifran ® CT) during the cultivation of anaerobic cultures resulted in the inhibition of bacterial population growth and division at a concentration of 25/250 µg/ml, with complete absence of bacterial development. Statistically significant inhibition of growth was observed at concentrations of 6.25/625 and 12.5/500 µg/ml. Conclusion. The use of automatic programmable cultivation methods for microbial populations of anaerobic periodontopathogenic bacteria, followed by growth curve analysis, demonstrated the superiority of the combined form of ciprofloxacin and tinidazole over the beta-lactam antibiotic amoxicillin with clavulanate sodium.