FIGURE 2 from Transcriptome Deconvolution Reveals Absence of Cancer Cell Expression Signature in Immune Checkpoint Blockade Response

DEGs and pathways between responders and nonresponders in the stroma. A, Heat map of genes consistently differentially expressed across discovery cohorts in the stroma. Heat maps are colored by the log₂ fold change of gene expression. P-values of individual cohorts are combined using the Fisher method, and the −log₁₀(meta P-value) is presented in the bar plot. Genes with q-value <0.01 and P-value <0.1 at least one ICB cohort are shown. B, Bar plots of deconvoluted stromal expression of the top DEGs. Error bars represent estimated SE. C, Pathway enrichment of DEGs in the stroma. Bar plot shows the −log₁₀(P-value) of significantly enriched pathways. Similar pathways are grouped by color. D, Network representation of enriched pathways. Large nodes represent enriched pathways. Pathways with high similarity are fused and shown in the same color. Small nodes represent genes associated with each pathway.