Microscopic and Biomolecular Result Diagnostic of Survey Filarias in the Community of Chicala Province of Bié Angola

bstract: Bié is referred to as an endemic location for filariasis and has never been selected for mass administration ivermectin.To understand the situation, we did a study in the locality of Chicala, in Kuito and evaluated the co-endemicity of human filariasis, to obtain data via calibrated thick droplet microscopy and subcutaneous biopsy of samples collected in Chicala in Kuito, Bié and samples collected and preserved on Wathman filter paper for molecular diagnosis of filariae; Material and methods; The research was approved by the ethics committee of the Agostinho Neto University medical school.in deliberation number: 17/2021 and the samples collected after free and informed consent, the universe was 6015 people, data obtained from the local administration and was selected probabilistically from an approximate value of n0 400, the sample of 320 participants distributed in 12 conglomerates where we did research prolectively; The field work involved 2 microscopes, to read the slides, 2 interviewers, 1 guide and 1 driver; The Oncocerca volvulus worm was isolated from subcutaneous cellular tissue extracted from the iliac crest and/or calf muscles and crushed on a glass slide, hydrated in 1 drop of saline and analyzed using a binocular microscope (HC Olympus®), with a 100x objective., regarding the presence or absence of the filaria Oncocerca volvulus and one drop of fresh blood was collected by digital puncture and applied to the slide in a smear and on top of the slide and stained with giemsa and analyzed under the Binocular microscope (HC Olympus®) for the presence or absence of the filariae Wuchereria bancrofty and Loa Loa.All survey data were entered into Microsoft Excel 2010 and IBM SPSS version 21 statistical software was used for analysis.Results The average age of participants in the study was 39.8 years, the findings found in the calibrated thick drop were 0.3%, (+) positive for Wuchereria