FIGURE 2 from Activation of the Mevalonate Pathway in Response to Anti-cancer Treatments Drives Glioblastoma Recurrences Through Activation of Rac-1

Upregulation of the mevalonate pathway in response to anti-cancer treatments in vivo is restricted to glioma cells. A, Schematic of the experimental design underlying Fig. 2. B–F, qRT-PCR for the cholesterol biosynthesis–related genes in the tumor specimen harvested from the PDOX GBM mouse model at day 2 after the treatment of radiation in combination with QTP (30 mg/kg) or TMZ (50 mg/kg) or ONC201 (50 mg/kg) or solvent control using the human-specific primers. G–K, qRT-PCR for the cholesterol biosynthesis–related genes in the tumor specimen harvested from the PDOX GBM mouse model at day 2 after the treatments using the mouse-specific primers. L, The levels of total, free cholesterol and cholesterol-esters in the tumors harvested from the PDOX GBM mouse model at day 2 after the treatments. M, The concentration of free fatty acid in the tumors harvested from the PDOX GBM mouse model at day 2 after the treatments. All experiments have been performed with at least three biological independent repeats. P-values were calculated using one-way ANOVA. ns: not significant.