Development of a Cu@ERGO-p(L-Lys) Modified GCE Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, and Uric Acid

In this work, a sensitive sensing platform was developed using a glassy carbon electrode (GCE) to simultaneously determine AA, DA, and UA. Cu nanostructures, the poly-L-Lysine ( p (L-Lys)), and the electrochemically reduced graphene oxide (ERGO) modified GCE (GCE/Cu@ERGO- p (L-Lys)) was developed via the electrodeposition of Cu and electropolymerization of the ERGO- p (L-Lys). Simultaneous detection makes analysis more efficient and cost-effective by reducing the need for multiple sensors. The GCE/Cu@ERGO- p (L-Lys) was characterized by electrochemical impedance spectroscopy, cyclic voltammetry, and scanning electron microscopy. The limit of detection values for AA, DA, and UA analytes were 0.16, 0.033, and 0.021 μ M, respectively, while the linear ranges were 0.53–50.0, 0.11–100.0, and 0.070–0.75 μ M. The proposed sensor was found to be applicable for the determination of target analytes in fetal bovine serum samples. The proposed GCE/Cu@ERGO- p (L-Lys) hybrid composite modified electrode is a promising material for simultaneous determination in biological fluids with excellent analytical performance and anti-interference effect.