Design, synthesis and evaluation of imidazo1,2-apyrazin-8(7H)-one derivatives as acetylcholinesterase inhibitors and antioxidants

Abstract A series of 8-(piperazin-1-yl)imidazo1,2-apyrazine derivatives were designed and synthesized as acetylcholinesterase inhibitors (AChEIs) and antioxidants for the treatment of Alzheimer's disease (AD). Moreover, the biological evaluation results demonstrated that these synthesized compounds exhibited moderate inhibitory activities toward acetylcholinesterase (AChE) and radical scavenging activities. Among them, compound 14r was the most potent AChE inhibitor with an IC50 value of 0.47 µM and moderate inhibitory activity against butyrylcholinesterase (BuChE) (IC50 = 11.02 µM). Meanwhile compound 14r had the best selectivity of AChE and selectivity index (SI) values was 23.45. Compound 14r has better activity as well as AChE selectivity compared to reference drug galantamine (AChE IC50 = 5.01 µM, BuChE IC50 = 18.46 µM, SI = 3.68). Compound 14o had the best antioxidant activity with an IC50 value of 89.33 µM, which was lower than that of ascorbic acid (IC50 value = 25.70 µM) as the control drug. Furthermore, the results of molecular docking studies indicated that 14r could simultaneously bind to both catalytic active site and peripheral anionic site of AChE, which was consistent with the mixed inhibition pattern shown by enzyme kinetic studies. The interaction’s stability of 14r-AChE/BuChE were also assessed using a conventional atomistic 100 ns dynamics simulation study, which revealed the conformational stability of representative compound 14r in the cavity of the AChE. In addition, the molecular properties of all compounds were predicted online through the SwissADME, and the best active compound 14r matched the properties of most orally administered drugs. Based on the biological activity and molecular properties, compound 14r as AChEI was valuable for further development.