Roles of amygdalin and celastrol on staurosporine-treated mesenchymal stem cells in an in vitro culture

Introduction: Substances of a plant origin have enjoyed widespread use in medicine for ages because they have a beneficial effect on the human body. Amygdalin and celastrol, which are obtained from plants, among others, have proven to have an anti-cancer and anti-inflammatory effect. The influence of these compounds on the survivability of stem cells in toxic conditions has not yet been studied.Objective: The aim of the study was to evaluate the effect of selected concentrations of amygdalin and celastrol on the level of survivability of mesenchymal stem cells (MSC) in the presence of a toxic substance – staurosporine.Methods: Cell viability WST-1 colorimetric assay.Results: In vitro, amygdalin and celastrol are cytotoxic to MSC at high doses. Amygdalin at a dose of 10 mM had a cytoprotective effect on the culture of MSC that had been exposed to staurosporine. Celastrol at doses of 0.25 and 1 M augmented the cytotoxic effect of staurosporine on the culture of MSC, although there was no statistical significance.