We present a method to map gene expression in formalin-fixed paraffin-embedded (FFPE) lung tissue sections using the 10x Genomics Visium Spatial FFPE v1 protocol. This approach utilizes whole-transcriptome probes to achieve precise localization of mRNA within tissue structures. By preserving tissue morphology and ensuring reliable detection, this protocol enables comprehensive studies of lung biology and pathology. The spatial transcriptomics data obtained offer valuable insights into gene regulation and cellular diversity, enhancing our understanding of lung tissue organization and disease mechanisms.
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Vanegas et al. (2024) studied this question.
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