In vitro inhibitory activities of indolealkaloid derivatives against porcine epidemic diarrhea virus

Abstract Porcine Epidemic diarrhea virus (PEDV) is an enteric coronavirus, which can cause acute diarrhea, vomiting, dehydration and high mortality of newborn piglets, and leading to huge economic losses to the world pig industry. Given the limited efficacy of current PEDV vaccines, there is an urgent need for the development of antiviral drugs. In this study, the antiviral effects of 17 synthesized indole alkaloid derivatives against PEDV were investigated. It was observed that the No.14 indole alkaloid derivative exhibited significant inhibition of PEDV replication in a dose-dependent manner. Furthermore, through time-of-addition assays and RT-qPCR, it was determined that delayed administration of the No.14 indole alkaloid derivative resulted in weaker inhibitory effects on PEDV compared to early treatment. Mechanistic analysis revealed that the No.14 indole alkaloid derivative exerts its inhibitory effects during the entry stage of the PEDV life cycle. This study demonstrates the anti-PEDV effects of the No.14 indole alkaloid derivative, suggesting its potential as a candidate drug for treating PEDV infections.