Imaging a large number of samples is necessary to improve statistical robustness in biological assays. Using standard multiwell plates is not possible in usual light-sheet microscopes. One solution is to use an Oblique Plane Microscope (OPM), which is a special fluorescence light sheet microscope, with a single objective near to the sample. To avoid aberrations, OPM generally uses water or silicon immersion to match the refractive index of the sample. In this work we present an oil-immersed OPM and experimentally demonstrate the possibility of using a primary objective with a higher numerical aperture.
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Bécar et al. (2024) studied this question.
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