Study on the optimizing determination of selenocysteine content based on fluorescence method

The determination of selenocysteine was investigated based on the fact that the -SeH bond in selenols can specifically undergo nucleophilic addition reaction with the Se-N bond in benzoselenadiazole. The results obtained showed that the pH of t determination system was 4.7 and the optimum temperature was 37°C. The linear relationship was F=90.246c+298.36, and the correlation coefficient R² was 0.9916. The relative error of this method was less than 2.2%, and the recoveries were in the range of 96.6%~103%. This experimental method is based on the nucleophilic addition reaction of -SeH, which enables the rapid and accurate determination of selenocysteine without catalysis, heating and long waiting time. This approach provides some reference for the study of the physiological significance of selenium.