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Microbiological and molecular PCR techniques were used to analyse 328 samples of mussels (Mytilus galloprovincialis) cultured in Istrian waters. Molecular testing was more effective than microbiological testing, detecting the presence of the bacterium Vibrio parahaemolyticus in 21.34% of samples, compared to 2.44% detected through microbiological methods. The probability of detecting the presence of this bacterium was 7.19 times higher when the sea surface temperature was above 15°C, while thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) positive samples were detected at sea temperatures above 22.5°C and 24.7°C, respectively. TDH- and TRH-positive samples constituted 10% of the total V. parahaemolyticus-positive samples. As there is no legal obligation to monitor the presence of this bacterium, consuming raw or insufficiently cooked mussels can lead to illness.
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Džafić et al. (2024) studied this question.
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