Determining ideal offsets of spatially offset Raman spectroscopy for transcutaneous measurements—A Monte Carlo study

Spatially offset Raman spectroscopy (SORS) is valuable for noninvasive bone assessment but requires a clearer understanding of how offset distances influence detection depth. To address this, our study devised a forward‐adjoint Monte Carlo multi‐layer (MCML) model to simulate photon paths in SORS, aiming to determine optimal offsets for various tissue types. We examined photon migration at offsets between 0 and 15 mm against layered phantoms of differing thicknesses and compositions to optimize the signal‐to‐noise ratio for bone layers. The findings highlight that optimal offsets are contingent on tissue characteristics: a metacarpal beneath 2.5 mm of tissue had an ideal offset of 6.7 mm, while a tibia with 5 mm of soft tissue required 10–11 mm. This precise calibration of SORS via MCML modeling promises substantial improvements in bone health diagnostics and potential for expansive medical applications.