Protocol to study the immune profile of syngeneic mouse tumor models

Protocol to study the immune profile of syngeneic mouse tumor models Flow cytometry, single-cell RNA sequencing, and other analyses enable us to capture immune profiles of the tumor microenvironment.Here, we present a protocol to characterize the immune profile of tumor-bearing mice.We describe steps for establishing mouse models and preparing single-cell suspensions from tumor tissue and other immune-related organs, which can be further analyzed by flow cytometry and other omics assays.We then detail procedures for staining, flow cytometry analysis, and phenotyping of the immune cell populations.