Figure 4 from Deuterium Metabolic Imaging Differentiates Glioblastoma Metabolic Subtypes and Detects Early Response to Chemoradiotherapy

Spectroscopic measurements of the effects of chemoradiation on glucose metabolism in tumor models representative of the glycolytic and mitochondrial subtypes. Serial 5-minute tumor ²H spectra were acquired following an i.v. injection of [6,6′-²H₂]glucose in two glycolytic subtypes of GBM (A11 and U87; A–H) and two mitochondrial subtypes (S2 and AT5; I–P) before and 24 hours after targeted chemoradiotherapy. Measurements of ²H-labeled lactate and Glx in serial 5-minute spectra acquired from tumor-bearing animals pre- and posttreatment of A11 (A and B), U87 (C and D), S2 (I and J), and AT5 (K and L) tumors. The concentrations of ²H-labeled lactate and Glx measured between 20 and 65 minutes following [6,6′-²H₂]glucose injection were compared pre- and posttreatment in A11 (E and F), U87 (G and H), S2 (M and N), and AT5 (O and P) tumor–bearing animals. ns, not significant; *, P < 0.05; ****, P < 0.0001.