The physicochemical and microbial attributes of storm water samples prior to and after mycofiltration was determined using routine methods. The preparation of the substrate was done by supplementation of un-fermented sawdust with calcium carbonate, calcium sulphate, granulated sugar and wheat offal. The mixture was allowed to undergo composting for 7 days upon which, it was inoculated with Lentinus squarrolsulus spawn and incubated at room temperature. The sample was then passed through a network of mycelia for pollutant removal. The mean TDS and EC concentrations of the raw and mycofiltered samples was 1369.8 ± 4.5 and 516.4 ± 2.9 mg/l as well as 2785.0 ± 4.2 and 1251.0 ± 5.6 µS/cm. Pb, Cu, Cd, Cr and Fe readings for the raw samples were 0.03±0.03mg/l, 0.07±0.03 mg/l, 0.01±0.01mg/l, 0.03±0.01mg/l and 1.00±0.11mg/l. For the mycofiltered samples, the Pb, Cd and Cr were reduced to nil while Cu and Fe decreased to 0.03 ± 0.03 mg/l and 0.47± 0.09 mg/l. The difference between the mean trace metal values recorded for the raw and filtered samples was insignificant (p>0.05). The results indicated that mycofilter derived from L. squarrolsulus mycelia was capable of purifying storm water sample.
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