FIGURE 4 from Nucleolar Localization of the RNA Helicase DDX21 Predicts Survival Outcomes in Gynecologic Cancers

DDX21 nucleolar localization associates with PARP1 expression and decreased survival outcomes. A and B, Representative immunohistochemical staining of PARP1 and DDX21 from endometrial (A) and ovarian (B) cancer commercial tissue microarrays (left) and box plots showing quantification of nucleolar DDX21 intensity in low versus high PARP1 endometrial (A) and ovarian (B) cancer samples (right). Student t test; ****, P < 0.0001. B, Representative IHC images from advanced stage endometrioid endometrial cancer patient samples showing low versus high nucleolar DDX21 staining by IHC. Kaplan–Meier curves showing high nucleolar localization of DDX21 is significantly associated with decreased OS (P-value = 0.0155; D) and PFS (P-value = 0.0092; E) in patients with advanced stage endometrioid endometrial cancer. Total n = 39 (number of patients with low DDX21 nucleolar staining = 14; number of patients with high DDX21 nucleolar staining = 25). log-rank (Mantel–Cox) test; *, P < 0.05; **, P < 0.01.