Impact of Prolonged Glycation Pre-Treatment on the Electron Transfer Kinetics of Cytochrome C

The effect of long term (up to 25 days) glycation process by D-glucose on the electron transfer properties of Cyt C under artificial conditions, using modified gold electrode, as a redox partner, has been studied. The redox kinetics of Cyt C, in the 0.1 M buffer solution containing 4 mg/mL Cyt C and 200 G/L D-glucose (T=25 0C), by the glycation time more than three weeks was directly measured using the method of cyclic voltammetry. Systems voltamperic response has shown, that redox activity of glycated Cyt C is preserved upon the glycation time, at least, during the incubation period up to 25 days.