FIGURE 1 from Epigenetic Priming by Hypomethylation Enhances the Immunogenic Potential of Tolinapant in T-cell Lymphoma

Effect of necrosome protein expression in TCL cell lines on tolinapant-induced cell death. A, Western blots of parental BW5147, RIPK3⁻/⁻ BW5147 and MLKL⁻/⁻ BW5147 cell lysates (untreated). B, Cytotox-NIR signal captured by real-time microscopy (IncuCyte), detecting membrane permeabilization during lytic cell death after treatment of parental BW5147, RIPK3⁻/⁻ BW5147 and MLKL⁻/⁻ BW5147 cells with tolinapant in the absence or presence of zVAD. C, Western blots of Karpas-299 cells transduced with control gRNA (KARPAS-299-CTR gRNA) or with RIPK3 gRNA (KARPAS-299-RIPK3 gRNA) constructs treated with tolinapant ± zVAD for 0, 2, or 6 hours. D, Real-time microscopy (IncuCyte) measurement of membrane permeabilization by measuring Cytotox-NIR signal after treatment of KARPAS-299-CTR gRNA or KARPAS-299-RIPK3 gRNA cells with tolinapant. E, Western blots of EL4-PAR and CASP8 KO EL4 (EL4-C8KO) cell lysates after 24-hour treatment with tolinapant. F, Real-time microscopy (IncuCyte) measurement of membrane permeabilization by measuring Cytotox-NIR signal after treatment of EL4-PAR or EL4-C8KO cells with tolinapant.